From the above results we can conclude that vitek 2 compact is an automated system and gives more accurate results. A previously healthy chinese male returned from working in the malaysian jungle with a fever. Keywords antimicrobial susceptibility, vitek2, pyogenic samples, resistance, staphylococcus, gram. Geneup system for foodborne pathogen testing duration. Administrator or supervisor level login access is required. This site is like a library, you could find million book here by using search box in the header. Vitek manual table of contents mount sinai hospital. Microbial identification using vitek 2 pdf pdadhi parenteral. The newly launched vitek 2 compact, a system for identification and antibiotic susceptibility testing ast, offers the same advantages as. The colorimetric vitek2 compact system achieved an excellent performance to provide accurate species id results. Vitek2 compact system is of great help to the clinicians as compared to the standard methods used, the use of vitek2 compact system would reduce the turnaround time by at least 16 hrs. Pdf vitek 2 automated identification system and kocuria. Vitek 2 compact bacterial identification and monitoring.
The system is now initialized and ready for data entry. Vitek 2 and vitek 2 compact systems using vitek 2 systems 8. Identification and antibiogram of various gram positive. The reliability of using vitek 2 compact system to detect. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of capacity and automation. This study compares the usefulness of the semiautomated vitek 2 compact system vs. Agreement and cost comparison of vitek2 compact, vitek2 60, vitek ms systems and api20ene using clinical gramnegative bacilli isolates from. For the love of physics walter lewin may 16, 2011 duration. All verifications were deemed acceptable by the laboratory director. The vitek 2 is an automated microbiology system utilizing growthbased technology.
This system allows kinetic analysis by reading each test every. Rapid reliable identification of these organisms is essential for accurate diagnosis and prompt effective treatment. No reagent addition needed with closed, readytouse disposable system. Comparative evaluation of the vitek 2 advanced expert system aes in five uk hospitals. The only strain unidentified by api id 32c was later identified. Pdf the reliability of using vitek 2 compact system to. Select vitek 2 compact to initiate the system from the upper left side of the screen. Vitek 2 xl vitek 2 compact vitek 2 0118 93192002gbe document andor pictures not legally binding. Observa is an information management system that stores the test data generated by the vitek 2 or vitek 2 compact. Vitek 2 compact, automated idast instrument biomerieux.
Evaluation of the vitek 2 system for identification and. After the system is initiated, log onto the system using the appropriate user name and password. Extendedspectrum betalactamases esbls are plasmidmediated betalactamases that are capable of hydrolysing. The information is then gathered and formatted into many vital reports, including epidemiology and statistical reports. Scan cassette worksheet at the work station vitek 2 is designed to make your idast workflow as rapid and reliable as possible, while providing maximum flexibility and full traceability. Inoculate organism into 3ml vitek saline tube, vortex and invert the tube 2 to 3 times to uniformly suspend inoculum. Us environmental protection agency office of pesticide. Subsequent verifications were performed when new drugs were added 11214 and 41415. A blood culture grew gramnegative bacilli that were initially identified as burkholderia cepacia by the vitek 2 system but were subsequently found to be burkholderia pseudomallei by partial sequencing of the 16s rrna gene. Performance of the vitek 2 system software version 5. The global increased prevalence of esblproducing bacteria creates an urgent need for. Biomerieux was also informed that the isolate organism belonged to a pt that died. Refer to the vitek 2 systems software user manual or the vitek 2 systems web software user manual for information about accessing, creating and managing user accounts. The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the vitek 2 instrument and the smart carrier station.
Pdf agreement and cost comparison of vitek2 compact. Evaluation of the vitek 2 system for identification and antimicrobial. Verification of antimicrobial susceptibility testing. The customer reported that the organism isolated from urine sample was identified, by the vitek 2 compact instrument, as b6 98% probability on b6 2011. A total of 405 clinically relevant nonduplicate staphylococcal isolates. These cultures were then used to make suspensions in 0. Designed to provide idast results in as little as 5 to 8 hours 3, vitek 2 compact works with economical, readytouse vitek 2 idast cards. Us federal law restricts this device to sale by or on the order of a licensed practitioner. The problematic isolate with lightgreen colonies was iden tified with api id 32c as c. Vitek 2 card a unique concept in idast designed to provide idast results in as little as 5 to 8 hours. Preamble for todays seminar the process of verifying an antimicrobial susceptibility testing ast system can be very confusing. In the present study 198 clinical isolates, including pseudomonas aeruginosa n 146, acinetobacter baumannii n 25, and stenotrophomonas maltophilia n 27 were evaluated. Customer training course book and instrument user manual are maintained near.
Select vitek 2 compact to initiate the system from the upper left. Several manual and commercial identification methods have been developed and are now routinely used 4, 5, 15, 23, 24, 25, 26, 31. Advances in life science and technology issn 22247181 paper issn 2225062x online vol. Vitek 2 compact identifies the vast majority of microorganisms that contaminate. The vitek system originated in the 1970s as an automated system for identification and ast and has evolved today into the vitek 2 system, which automatically performs all of the steps required for identification and ast after a primary inoculum has been prepared and standardized. Prep station entering target slide and vitek 2 ast test cards duration. Subculture frozen or freeze dried isolates twice before setting up any vitek 2 cards. Reference susceptibility testing of cefepime, cefotaxime, ceftazidime, ciprofloxacin. The instrument was received and the test system verified in 2012. This documentation is filed in vitek2 verification files. Evaluation of the vitek 2 system for the identification. The vitek 2 advanced expert system software is like having an expert advisor standing by your side. Place assembled card and transfer tube on filling stand with the long portion of the transfer tube into the test tube. Click the vitek 2 system legacy icon on the desktop.
Read online vitek 2 compact manual book pdf free download link book now. Laboratorybased evaluation of the colorimetric vitek2. The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana henry kwadwo hackman1, george osei. Use of the vitek 2 system for rapid identification of. Staphylococci are an increasing cause of bloodstream infections. The vitek 2 advanced expert system software is like having an. Usefulness of chromagar candida medium, biochemical. The innovative vitek 2 technology includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal training time. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of. In addition, there are several different reasons why. In the present study, the vitek 2 system was compared with conventional methods for the identification of yeasts in clinical specimens. Modifications by biomerieux can be made without prior notice biomerieux, the blue logo, advanced expert system, bioart, densichek, myla, vilink and vitek are used, pending andor registered trademarks belonging to biomerieux, or one of its subsid iaries, or one of its. This chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Principles the vitek 2 is an automated microbiology system utilizing growthbased technology.
Vitek 2 instrument user manual i 51073110en1 general warnings important. Vitek 2 technology represents a smarter way to automate idast testing. The reliability of using vitek 2 compact system to detect 1. The configuration that you have purchased is adapted to the legislation.
Safety is optimized with minimal reagent preparation and reduced handling in a closed, disposable system. Comparison of the microscan walkaway and vitek 2 compact. Vitek 2 is a new automatic system for the identification and susceptibility testing of the most clinically important bacteria. Place a small barcode label near the top of a 3ml vitek saline tube. Table 1 descriptive measures from the automated system vitek 2 compact. Download vitek 2 compact manual book pdf free download link or read online here in pdf.